During my Ph.D. work I studied some of the mechanisms that regulate the complex machinery of vesicular transport from the endoplasmic reticulum to the Golgi apparatus in the single-celled eukaryote Saccharomyces cerevisiae. I focused my attention on certain proteins involved in vesicular budding, particularly on Sec24p family members. In addition I studied proteins involved in tethering/docking processes, in particular a new member of the Gyp family of GTPase activating proteins (GAP), Gyp5p, the preferred substrate of which is the small Ras-like Ypt1 protein.
Structural and functional analysis of yeast proteins involved in ER-to-Golgi transport: Sec24p family proteins and the GTPase activating protein Gyp5p
ISBN : 978-3-89653-874-1